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NAMRATA PUNIT AWASTHI

RAM MANOHAR LOHIA INSTITUTE OF MEDICAL SCIENCES, India

Title: Structural parameters for WBC in Adult Sepsis

Biography

Biography: NAMRATA PUNIT AWASTHI

Abstract

Background and Purpose:  Newer hematology analyzers offer structural parameters for WBC (SPW) obtained from a routine blood count and can provide quantitative information about the inflamma­tory reaction of the patient’s immune system. SPW obtained from Sysmex XN1000 automated hematology analyzer were evaluated as prospective biomarkers for sepsis diagnosis in adult patients.

Methodology: Adult patients admitted in (ICU n=52) were included in the study. Acute change in SOFA score of 2/more with clinical suspicion and/or culture positivity formed the basis of sepsis diagnosis. Whole blood sample in EDTA for blood counts was collected within 24hours of clinical suspicion for infection. Other investigations were performed as per the standard protocol in ICU. Following structural WBC parameters related to side scatter, side fluorescence, forward scatter and distribution width obtained from Sysmex XN1000 analyzer were evaluated: NE-SSC, NE-SFL, NE-FSC, MO-X, MO-Y, MO-Z, MO-WX, MO-WY and MO-WZ. Data was analyzed on SPSS software and represented as mean±SD. Difference in mean calculated by unpaired t test.

Results and discussion: Cases were categorized into Sepsis n=27 and Disease control n=25. Mean ±SD (p) for NE-SFL(neutrophil fluorescence) and NE SSC(neutrophil side scatter) in sepsis vs. disease control were 69.62±7.10 vs. 60.11±6.12( p=0.000) and 155.76±3.24vs. 151.58±5.44(p=0.002) respectively. For Monocyte parameters, only MO-X(Monocyte side scatter) was significantly different in both the groups (125.66±4.60 vs 120.81±3.12, p=0.000).

Conclusion: We observed that certain SPW revealed remarkable changes in patients of sepsis as compared to non-sepsis patients admitted in ICU. A combination of SPW along with biomarkers of sepsis like Procalcitonin and QCRP may add valuable information for early diagnosis of sepsis before culture results.