Mehmet Rıfat Vural
Ankara University, Turkey
Title: Investigation of relationship between expressions of TGF-β family members, GATAs, IGFs and apoptosis in bovine ovarian cysts
Biography
Biography: Mehmet Rıfat Vural
Abstract
The mechanism of bovine ovarian cysts is not completely understood. The role of locally produced intraovarian or intrafollicular factors have been discussed at the transformation from growing follicule into cystic structure. Local expression of proteins or factors, including transforming growth factor family members (TGF-β), growth factors and transcription factors, may play a central role as intraovarian regulator of folliculogenesis in mammals. The objective of the study was to reveal of relationship between role of TGF- β family members (AMH, GDF-9, BMP-6, Inhibin-α), transcriptor factors (GATA-4, GATA-6), and growth factors (IGF-I, IGF-II) expressions and apoptosis in bovine ovarian cysts. Cystic ovaries were collected from slaughterhouses. After macroscopical evaluation, cysts in both ovarii were subclassified histopathologically into follicular (types II-III) cyst and luteal cyst. Immunperoxidase method elucidated in follicular cyst type I that mild reactions by IGF-I, IGF-II, GATA-4 and inhibin-α were present in superficial and basal epithelial cells, by GATA-4 and GATA-6 in theca interna and externa cells, by IGF-II and GATA-4 in stroma. In follicular cyst type II, positivities varying degree from mild to moderate reactions by the all markers were observed in epithelial cells and theca cells and by other markers excluding IGF-I and II in stroma. In follicular cyst type III, mild or moderate reactions by all markers were obtained in stroma excepting for IGF-I. TUNEL positivities were densed in generally epithelial cells and sometimes theca cells of cyst type II and III. We believe that TGF- β superfamily members and IGFs may trigger cystic degeneration and indirectly apoptosis as a result of genetic and environmental factors.
